|recombinant human EGF protein (Qk011)||£50.00 – £175.00|
Human EGF protein is a potent EGF-family growth factor used in many human and mouse organoid and stem cell culture systems including intestinal and tumor (cancer) organoid culture.
A pure 6.3 kDa biologically active mature human epidermal growth factor. Our recombinant EGF protein is manufactured to the highest purity in E. coli and extensively tested, making it ideal for use in chemically defined iPSC, ESC and organoid culture media.
|Show details||ipsc-esc-differentiation egf endodermal epithelial ipsc-esc-maintenance neural organoid|
|recombinant human FGF-2 / bFGF protein (145 aa) (Qk025)||£100.00 – £600.00|
Human FGF2 /bFGF protein (145 aa) is our gold-standard and recommended FGF2 / bFGF protein for induced pluripotent stem cell (iPSC) and embryonic stem cells (ESC) maintenance, and induced pluripotent and mesenchymal stem cells proliferation and differentiation.
High purity and bioactivity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF) comprising the core structured region (145 amino acids) sufficient for full biological activity.
|Show details||ipsc-esc-differentiation fgfs ipsc-esc-maintenance organoid|
|recombinant human FGF-2 /bFGF protein (154 aa) (Qk027)||£100.00 – £600.00|
Human FGF2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein. Human bFGF / FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154aa form of FGF2 comprises the core structured region and N-terminal extension.
High purity and bioactivity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).
|Show details||ipsc-esc-differentiation fgfs ipsc-esc-maintenance organoid|
|recombinant human R-spondin 3 protein (Qk032)||£120.00 – £1,600.00|
Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.
17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.
|Show details||ipsc-esc-differentiation optimised-by-qkine organoid r-spondins|
|recombinant mouse LIF protein (Qk018)||£120.00 – £1,600.00|
Mouse LIF (murine leukemia inhibitory factor) protein supports the derivation, propagation and expansion of mouse embryonic stem cells (ESC), and maintenance of pluripotency. LIF protein increases the robustness of mouse embryonic stem cell culture in feeder or feeder-free chemically defined culture systems.
Qkine recombinant mouse LIF protein is animal-free and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression (1).
|Show details||ipsc-esc-maintenance lifs mouse-es-cell|
|recombinant human LIF protein (Qk036)||£120.00 – £1,600.00|
Human LIF (leukemia inhibitory factor) protein suppresses the differentiation of embryonic stem cells (ESCs) and is widely used in ESC and iPSC culture (1). Human LIF can be used in the maintenance of both murine and human ESCs in feeder or feeder-free chemically defined culture systems (2).
LIF is a pleiotrophic factor that belongs to the IL-6 superfamily of cytokines. Acting through a heterodimeric receptor of gp130 and gp190, LIF can activate a number of cellular pathways including the JAK/STAT, PI3K and MAPK pathways (3).
Qkine human LIF protein is high purity and animal and carrier-protein free for reproducible results.
|Show details||ipsc-esc-maintenance lifs mouse-es-cell neural|
|recombinant human FGF-9 protein (Qk039)||£120.00 – £1,600.00|
Human FGF-9 protein is a member of the fibroblast growth factor (FGF) family, FGF-9 is involved in many developmental processes including neural and skeletal development. Recombinant FGF9 is used frequently in the generation of kidney organoids.
Highly pure and bioactive 23 kDa FGF-9 protein, animal-derived component free (ACDF) and carrier-protein free (CF)
|Show details||fgfs mesenchymal neural organoid|
|recombinant human/mouse/rat follistatin-resistant activin A protein (Qk035)||£120.00 – £180.00|
Follistatin-resistant activin A (FRACTA) has been engineered to prevent binding to the natural inhibitor, follistatin. Activin A activity protein is regulated in vivo by follistatin, a high-affinity inhibitor; follistatin accumulates in stem cell culture, where it inhibits activin A.
Qk035 follistatin-resistant activin A (FRACTA) has equivalent bioactivity to wild-type activin A (Qk001) but does not bind follistatin so is resistant to feedback inhibition. High purity 26 kDa dimer comprising engineered mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF). This specialised activin A has been developed in Marko Hyvönen’s group in the University of Cambridge.
|Show details||ipsc-esc-differentiation ipsc-esc-maintenance optimised-by-qkine pioneering-proteins|
|recombinant human R-spondin 1 LR5 protein (Qk031)||£120.00 – £750.00|
Recombinant R-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor. In epithelial tissues LGR5 marks the stem cell population. This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population. R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells. This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).
|Show details||ipsc-esc-differentiation ipsc-esc-maintenance optimised-by-qkine organoid pioneering-proteins r-spondins|
|recombinant human Gremlin 1 protein (Qk015)||£160.00 – £1,200.00|
Human Gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to Noggin for optimisation of intestinal organoid culture and iPSC differentiation.
Qk015 has been optimized by our experts for exceptionally high-purity production in E.coli and bioactivity. 18 kDa high purity dimeric protein, animal-free and carrier protein free
|Show details||ipsc-esc-differentiation bmps ipsc-esc-maintenance optimised-by-qkine organoid|
|recombinant human HGF (NK1) protein (Qk013)||£160.00 – £1,200.00|
Human HGF (NK1) protein is a potent, high purity NK1 isoform of human hepatocyte growth factor (HGF) that promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α. The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.
20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
|Show details||ipsc-esc-differentiation hgf optimised-by-qkine organoid|
|recombinant human FGF-4 protein (Qk004)||£160.00 – £1,200.00|
Human FGF4 protein is used for the proliferation and differentiation of embryonic and induced-pluripotent and tissue (mesenchymal) stem cells and promotes neural stem cell proliferation. FGF4 is an important component of cardiac, intestinal and other organoid culture media.
High purity and bioactivity 14 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 4, animal-derived component free (ACDF) and carrier-protein free (CF).
|Show details||ipsc-esc-differentiation fgfs ipsc-esc-maintenance|
|recombinant human FGF-10 protein (Qk003)||£160.00 – £1,200.00|
Human FGF10 protein promotes lung organoid formation and induces branching morphology. FGF10 protein is used widely in organoid culture, embryonic stem cell (ESC) and induced-pluripotent stem cell (iPSC) differentiation, and for the study of epithelial to mesenchymal transition and tumor metastasis.
High purity and bioactivity 17 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 10, animal-derived component free (ACDF) and carrier-protein free (CF).
|Show details||ipsc-esc-differentiation fgfs organoid|
|recombinant mouse Noggin protein (Qk033)||£160.00 – £2,400.00|
Mouse Noggin supports growth of organoids derived from mouse epithelial tissues and stem cell differentiation. Noggin protein is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids; maintenance of embryonic stem cells and differentiation into neural lineages.
Qkine recombinant mouse noggin protein is a highly pure 46kDa bioactive dimer for reproducible results (animal-free and carrier-protein free).
|Show details||ipsc-esc-differentiation bmps epithelial ipsc-esc-maintenance mouse-es-cell neural noggins organoid|
|recombinant human noggin protein (Qk034)||£160.00 – £2,400.00|
Human noggin supports growth of organoids derived from epithelial tissues and iPSC and ESC differentiation. Noggin protein is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids; epithelial organoid culture (WNR media), maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.
Qkine human noggin protein is a highly pure 46kDa bioactive dimer for reproducible results in organoid culture.
|Show details||ipsc-esc-differentiation bmps ipsc-esc-maintenance mouse-es-cell neural noggins organoid|
|recombinant human R-spondin 1 protein (Qk006)||£170.00 – £1,600.00|
Human R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signalling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.
13kDa highly pure, bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Qk006 is animal-free and not glycosylated to ensure production of homogeneous protein and enhanced comparability between batches. Used to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.
|Show details||ipsc-esc-differentiation ipsc-esc-maintenance optimised-by-qkine organoid r-spondins|
|recombinant human/mouse/rat Activin A PLUS (Qk005)||£180.00 – £1,900.00|
Human/mouse/rat activin A PLUS protein is an optimised biologically active truncation of the mature domain of human Activin A protein. The EC50 and activity in stem cell culture of activin A PLUS is equivalent to the full mature domain activin A.
High purity 24 kDa dimer comprising truncated mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF). Recombinant activin A PLUS is designed to be manufactured at scale for cost-effective, large-scale stem cell culture applications.
|Show details||ipsc-esc-differentiation ipsc-esc-maintenance optimised-by-qkine|
|recombinant human TGF-β1 PLUS protein (Qk010)||£200.00 – £3,000.00|
Human TGF-β1 PLUS protein is the first entirely animal-free recombinant human transforming growth factor beta 1 (TGF-β1) protein for highly reproducible results and compatible with chemically-defined stem cell media. TGFβ1 is used extensively in E8-style induced pluripotent (iPSC) and embryonic (ESC) stem cell media.
High purity 24 kDa dimer comprising optimised mature domain of TGFβ1 protein, animal-free (AF) and carrier-protein free (CF). Our TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.
*Benchmark our TGF-β1 PLUS against your existing supplier*
|Show details||ipsc-esc-maintenance optimised-by-qkine|
|recombinant human BMP-4 protein (Qk038)||£200.00 – £450.00|
Human BMP4 (bone morphogenetic protein 4) protein is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP4 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.
Highly pure 26 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP4 protein (animal-free and carrier protein-free).
|Show details||ipsc-esc-differentiation bmp4 bmps epithelial ipsc-esc-maintenance mesenchymal neural organoid|
|recombinant human/mouse/rat activin A protein (Qk001)||£240.00 – £2,400.00|
Human/mouse/rat activin A protein is a member of the TGFβ superfamily of growth factors used for maintenance of pluripotency in many induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) lines, and to induce stem cell differentiation into endoderm and other cell fates. Extensively validated for its reproducibility and as used at the Cambridge Stem Cell Institute.
High purity 26 kDa dimer comprising mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF). Rigorously benchmarked against other commercial sources. We’re proud of it … what do you think? A commentary by Qkine’s founder Marko Hyvönen.
|Show details||ipsc-esc-differentiation ipsc-esc-maintenance|
|recombinant human BMP-2 protein (Qk007)||£240.00 – £2,400.00|
Human BMP2 protein (bone morphogenetic protein 2) protein is member of the TGFβ family and a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.
26 kDa disulfide–linked bioactive highly pure dimer comprised of the mature domain of human BMP2 protein (animal-free and carrier protein-free). Our recombinant human BMP2 protein has been optimized for expression in E. coli and purified to homogeneity, offering a highly reliable and cost-effective reagent for the regulation of stem cell differentiation.
|Show details||ipsc-esc-differentiation bmps|
|recombinant human GDF-15 protein (Qk017)||£240.00 – £2,400.00|
Human GDF15 (growth differentiation factor 15) protein is a member of the TGFβ family and subject of intense interest as a marker of cellular stress and for its role in metabolism, cancer and pregnancy. Human GDF15 also is functional in mouse studies.
This 25 kDa disulfide-linked dimer is composed of the mature domain of human growth differentiation factor 15 protein. Our recombinant GDF15 protein is expressed in E. coli and exceptionally high purity extensively validated to ensure no trace contamination of related TGFβ family proteins from the mammalian culture systems, which historically led to controversy in the literature.
|Show details||biomarkers gdf15|
|recombinant zebrafish FGF-2 / bFGF protein (Qk002)||£250.00 – £400.00|
Zebrafish FGF2 protein (bFGF/basic FGF) 2 has been used extensively to support the maintenance and proliferation of human and mouse induced pluripotent (iPSC) and embryonic stem cells (ESC); used in the original Ludwig et al feeder-free culture of embryonic stem cells protocols 1-3.
High purity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF). This version of recombinant zebrafish FGF2 is used by the core facility at the Cambridge Stem Cell Institute, UK and historically by many of the stem cell groups based at University of Cambridge.
|Show details||ipsc-esc-differentiation fgfs ipsc-esc-maintenance organoid|