To enhance reproducibility and support scale-up, all growth factors and cytokines are:
- High purity and bioactive
- Carrier protein free
- Animal-origin free (AOF) or animal-derived component free (ADCF)
Image | Name | Price | Summary | Quick View | hf:categories |
---|---|---|---|---|---|
recombinant human TGF-β1 PLUS protein (Qk010) | £200.00 – £3,000.00 | Recombinant human TGF-β1 PLUS protein is the first entirely animal-free recombinant human transforming growth factor beta 1 (TGF-β1) protein for highly reproducible results and compatible with chemically-defined stem cell media. Our TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK. *Benchmark our TGF-β1 PLUS against your existing supplier* | ipsc-esc-maintenance optimised-by-qkine | ||
recombinant human R-spondin 3 protein (Qk032) | £170.00 – £1,600.00 | Recombinant human R-spondin 3 protein is a 17 kDa bioactive fragment of human R-spondin 3 protein comprised of the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signal potentiation and are the essential domains for activity in stem cell and organoid culture. The glycosylation of our recombinant human R-spondin 3 protein is unnecessary for extracellular signalling and stability. Our methods allow for optimized reliable production of homogeneous protein and enhanced comparability between batches. We provide bulk pricing and batch stock reservation for this protein. | ipsc-esc-differentiation optimised-by-qkine organoid r-spondins | ||
recombinant human R-spondin 1 LR5 protein (Qk031) | £120.00 – £750.00 | Recombinant R-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor. In epithelial tissues LGR5 marks the stem cell population. This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population. R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells. This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge). | ipsc-esc-differentiation ipsc-esc-maintenance optimised-by-qkine organoid r-spondins | ||
recombinant human R-spondin 1 protein (Qk006) | £170.00 – £1,600.00 | Recombinant human R-spondin 1 protein (RSPO1) is a 13kDa highly pure, bioactive fragment of human R-spondin 1. Our recombinant human R-spondin 1 protein is animal-free and not glycosylated to ensure production of homogeneous protein and enhanced comparability between batches. | ipsc-esc-differentiation ipsc-esc-maintenance optimised-by-qkine organoid r-spondins | ||
recombinant mouse Noggin protein (Qk033) | £160.00 – £2,400.00 | Recombinant mouse Noggin supports growth of organoids derived from mouse epithelial tissues and stem cell differentiation. Noggin protein is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids; maintenance of embryonic stem cells and differentiation into neural lineages. Qkine recombinant mouse noggin protein is animal and carrier-protein free for highly reproducible results and use in chemically defined media. | ipsc-esc-differentiation bmps epithelial ipsc-esc-maintenance mouse-es-cell neural noggins organoid | ||
recombinant human Noggin protein (Qk034) | £160.00 – £2,400.00 | Recombinant human Noggin supports growth of organoids derived from epithelial tissues and iPSC and ESC differentiation. Noggin protein is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids; epithelial organoid culture (WNR media), maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages. Qkine human noggin protein is high purity and animal and carrier-protein free for reproducible results and use in chemically defined stem cell and organoid culture media. | ipsc-esc-differentiation bmps ipsc-esc-maintenance mouse-es-cell neural noggins organoid | ||
recombinant mouse LIF protein (Qk018) | £100.00 – £1,600.00 | Recombinant mouse LIF (murine leukemia inhibitory factor) protein supports the derivation, propagation and expansion of mouse embryonic stem cells (ESC), and maintenance of pluripotency. LIF protein increases the robustness of mouse embryonic stem cell culture in feeder or feeder-free chemically defined culture systems. Qkine recombinant mouse LIF protein is animal and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression (1).
| ipsc-esc-maintenance lifs mouse-es-cell | ||
recombinant human LIF protein (Qk036) | £100.00 – £1,600.00 | Recombinant human LIF (leukemia inhibitory factor) protein suppresses the differentiation of embryonic stem cells (ESCs) and is widely used in ESC and iPSC culture (1). Human LIF can be used in the maintenance of both murine and human ESCs in feeder or feeder-free chemically defined culture systems (2). LIF is a pleiotrophic factor that belongs to the IL-6 superfamily of cytokines. Acting through a heterodimeric receptor of gp130 and gp190, LIF can activate a number of cellular pathways including the JAK/STAT, PI3K and MAPK pathways (3). Qkine human LIF protein is high purity and animal and carrier-protein free for reproducible results. | ipsc-esc-maintenance lifs mouse-es-cell neural | ||
recombinant human HGF (NK1) protein (Qk013) | £160.00 – £1,200.00 | Recombinant human HGF (NK1) protein is a high purity NK1 isoform of human hepatocyte growth factor (HGF) that promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α. The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up. | ipsc-esc-differentiation hgf optimised-by-qkine organoid | ||
recombinant human Gremlin 1 protein (Qk015) | £160.00 – £1,200.00 | Recombinant human Gremlin 1 protein has been optimized by our experts for exceptionally high-purity production and bioactivity. Gremlin-1 is a BMP-inhibitor present in the natural intestinal niche and provides an animal-free substitute for Noggin in chemically-defined media for many ESC, iPSC and organoid culture systems. | ipsc-esc-differentiation bmps ipsc-esc-maintenance optimised-by-qkine organoid | ||
recombinant human GDF-15 protein (Qk017) | £240.00 – £2,400.00 | Recombinant human GDF15 (growth differentiation factor 15) protein is a 25 kDa disulfide-linked dimer composed of the mature domain of human growth differentiation factor 15 protein. Our recombinant GDF15 protein is expressed in E. coli and extensively validated to ensure no trace contamination of TGFβ, which can cause misleading results. | biomarkers gdf15 | ||
recombinant human FGF4 protein (Qk004) | £160.00 – £1,200.00 | Recombinant human FGF4 protein is a 14 kDa non-glycosylated, high purity, bioactive domain of human fibroblast growth factor 4 expressed in E. coli. For the proliferation and differentiation of embryonic and tissue stem cells and organoid culture. | ipsc-esc-differentiation fgfs ipsc-esc-maintenance | ||
recombinant zebrafish FGF-2 / bFGF protein (Qk002) | £250.00 – £400.00 | Recombinant zebrafish FGF2 protein (bFGF) is a 17 kDa biologically active domain of zebrafish fibroblast growth factor 2 protein. Recombinant zebrafish fibroblast growth factor 2 has been used extensively to support the maintenance of human stem cells1-3. This version of recombinant zebrafish FGF2 is used by the core facility at the Cambridge Stem Cell Institute, UK and historically by many of the stem cell groups based at University of Cambridge. | ipsc-esc-differentiation fgfs ipsc-esc-maintenance organoid | ||
recombinant human FGF-2 protein (154 aa) (Qk027) | £100.00 – £600.00 | Recombinant human FGF2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein. Human bFGF / FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154aa form of FGF2 comprises the core structured region and N-terminal extension. | ipsc-esc-differentiation fgfs ipsc-esc-maintenance organoid | ||
recombinant human FGF-2 protein (145 aa) (Qk025) | £100.00 – £600.00 | Recombinant human FGF2 /bFGF protein (145 aa) is highly bioactive human fibroblast growth factor 2 (basic FGF) protein. Our high-purity FGF2 protein is a reliable option for ES cell and iPSC maintenance, as well as induced pluripotent and mesenchymal stem cells proliferation and differentiation. Fibroblast growth factor 2 (FGF2) or basic FGF has a broad range of physiological roles including regulation of cell proliferation and differentiation. | ipsc-esc-differentiation fgfs ipsc-esc-maintenance organoid | ||
recombinant human FGF-10 protein (Qk003) | £160.00 – £1,200.00 | Recombinant human FGF10 protein is a 17 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 10. Our high-purity FGF10 protein is produced in E. coli and purified to homogeneity. For use in organoid culture, ESC/iPSC differentiation, and for the study of epithelial to mesenchymal transition and tumor metastasis. | ipsc-esc-differentiation fgfs organoid | ||
recombinant human EGF protein (Qk011) | £50.00 – £175.00 | Recombinant human EGF protein is a 6.3 kDa biologically active domain of human epidermal growth factor. Our recombinant EGF protein is expressed to the highest purity in E. coli and extensively tested, making it ideal for use in chemically defined iPSC, ESC and organoid culture media. | ipsc-esc-differentiation egf endodermal epithelial ipsc-esc-maintenance neural organoid | ||
recombinant human BMP-4 protein (Qk038) | £200.00 – £450.00 | Recombinant human BMP4 (bone morphogenetic protein 4) protein is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP4 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development. | ipsc-esc-differentiation bmp4 bmps epithelial ipsc-esc-maintenance mesenchymal neural organoid | ||
recombinant human BMP2 protein (Qk007) | £240.00 – £2,400.00 | Recombinant human BMP2 protein is a 26 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP2 protein. Our recombinant human BMP2 protein has been optimized for expression in E. coli and purified to homogeneity, offering a highly reliable and cost-effective option for the regulation of stem cell differentiation. | ipsc-esc-differentiation bmps | ||
recombinant human follistatin-resistant activin A protein (Qk035) | £120.00 – £180.00 | Recombinant follistatin-resistant activin A (FRACTA) has been engineered to prevent binding to the natural inhibitor, follistatin. Activin A protein is a member of the TGFβ superfamily of growth factors used for maintenance of pluripotency in many induced-pluripotent stem cell and embryonic stem cell lines, and to induce stem cell differentiation into endoderm and other cell fates. Activin A activity protein is regulated in vivo by follistatin, a high-affinity inhibitor; follistatin accumulates in stem cell culture, where it inhibits activin A. Qk035 follistatin-resistant activin A (FRACTA) has equivalent bioactivity to wild-type activin A (Qk001) but is unaffected by follistatin feedback. This specialised activin A has been developed in Marko Hyvönen’s group in the University of Cambridge. | ipsc-esc-differentiation ipsc-esc-maintenance optimised-by-qkine pioneering-proteins | ||
recombinant human Activin A protein (Qk001) | £240.00 – £2,400.00 | Recombinant human activin A protein is a 26 kDa disulfide-linked dimer composed of the mature active domain of human activin A protein. Our activin A protein has been extensively validated for its reproducibility in stem cell culture, and benchmarked against other commercial sources. We’re a little bit proud of it … what do you think? A commentary by Qkine’s founder Marko Hyvönen. | ipsc-esc-differentiation ipsc-esc-maintenance | ||
recombinant human Activin A PLUS (Qk005) | £180.00 – £1,900.00 | Recombinant human Activin A Plus protein is a high purity, animal-free 24 kDa biologically active truncation of the mature domain of human Activin A protein. Our recombinant Activin A PLUS is designed to be manufactured at scale for cost-effective large-scale stem cell culture applications. | ipsc-esc-differentiation ipsc-esc-maintenance optimised-by-qkine |