Recombinant human TGF-β1 PLUS protein is the first entirely animal-free recombinant human transforming growth factor beta 1 (TGF-β1) protein for highly reproducible results and compatible with chemically-defined stem cell media. Our  TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.

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Recombinant human R-spondin 3 protein is a 17 kDa bioactive fragment of human R-spondin 3 protein comprised of the two cysteine-rich furin-like domains, which are  necessary and sufficient for Wnt signal potentiation and are the essential domains for activity in stem cell and organoid culture.  The glycosylation of our recombinant human R-spondin 3 protein is unnecessary for extracellular signalling and stability. Our methods allow for optimized reliable production of homogeneous protein and enhanced comparability between batches. We provide bulk pricing and batch stock reservation for this protein.

Recombinant R-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor.  In epithelial tissues LGR5 marks the stem cell population.  This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population.  R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells.  This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).

Recombinant human R-spondin 1 protein (RSPO1) is a 13kDa highly pure, bioactive fragment of human R-spondin 1. Our recombinant human R-spondin 1 protein is animal-free and not glycosylated to ensure production of homogeneous protein and enhanced comparability between batches. 

Recombinant mouse Noggin supports growth of organoids derived from mouse epithelial tissues and stem cell differentiation. Noggin protein is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids; maintenance of embryonic stem cells and differentiation into neural lineages. Qkine recombinant mouse noggin protein is animal and carrier-protein free for highly reproducible results and use in chemically defined media.

Recombinant human Noggin supports growth of organoids derived from epithelial tissues and iPSC and ESC differentiation. Noggin protein is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids; epithelial organoid culture (WNR media), maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.

Qkine human noggin protein is high purity and animal and carrier-protein free for reproducible results and use in chemically defined stem cell and organoid culture media.

Recombinant mouse LIF (murine leukemia inhibitory factor) protein supports the derivation, propagation and expansion of mouse embryonic stem cells (ESC), and maintenance of pluripotency.   LIF protein increases the robustness of mouse embryonic stem cell culture in feeder or feeder-free chemically defined culture systems.

Qkine recombinant mouse LIF protein is animal and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression (1).

Recombinant human HGF (NK1) protein is a high purity NK1 isoform of human hepatocyte growth factor (HGF) that promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α.  The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up. 

Recombinant human FGF2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein.   Human bFGF / FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154aa form of FGF2 comprises the core structured region and N-terminal extension.

Recombinant human FGF2 /bFGF protein (145 aa) is highly bioactive human fibroblast growth factor 2 (basic FGF) protein. Our high-purity FGF2 protein is a reliable option for ES cell and iPSC maintenance, as well as induced pluripotent and mesenchymal stem cells proliferation and differentiation.

Fibroblast growth factor 2 (FGF2) or basic FGF has a broad range of physiological roles including regulation of cell proliferation and differentiation.

Recombinant human BMP4 (bone morphogenetic protein 4) protein is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP4 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.

Recombinant human BMP2 protein is a 26 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP2 protein. Our recombinant human BMP2 protein has been optimized for expression in E. coli and purified to homogeneity, offering a highly reliable and cost-effective option for the regulation of stem cell differentiation.

Recombinant human Activin A Plus protein is a high purity, animal-free 24 kDa biologically active truncation of the mature domain of human Activin A protein. Our recombinant Activin A PLUS is designed to be manufactured at scale for cost-effective large-scale stem cell culture applications.