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R-Spondin-3 (RSPO3) protein is an essential component of Wnt 3a, R-spondin-3 (or 1,2), Noggin (WRN) media used widely in propagation of intestinal organoids and other stem cell and organoid models. Animal and carrier protein free.
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Human recombinant FGF2 protein 154 amino-acids for stem cell culture. Animal and carrier protein free.
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Mature domain of human FGF2 (residues 144-288, Uniprot: P09038) expressed in E.coli and purified to homogeneity. Protein is in PBS at 1 mg/ml (carrier protein-free)
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Mature domain of recombinant human GDF15 protein (Uniprot: Q99988) expressed in E.coli. No contaminating TGF beta family growth factors. Protein is lyophilised (carrier protein-free).
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Recombinant human Gremlin-1 protein. BMP2 and BMP4 inhibitor used in stem cell and organoid culture. Animal-derived component free and carrier protein free
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Human recombinant HGF NK1 protein for stem cell culture. Animal and carrier protein free.
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Epidermal growth factor (EGF) recombinant protein for induced-pluripotent stem cell (iPSC), embryonic stem cell (ESC) and organoid culture. For successful expansion and differentiation of epithelial, neural, mesoderm and hematopoietic lineages. Use along with other stem cell niche factors, R-spondin 1, Noggin or Gremlin, Wnt3a and FGF10, in defined and animal-derived component free media for establishing and maintaining intestine, stomach, liver, pancreas, brain and cancer organoids.
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Mature domain of human BMP-2 (residues 311-426, Uniprot: P08476) expressed in E.coli, refolded and purified to homogeneity. Protein is lyophilised (carrier protein-free)
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Receptor-binding active domain of human R-spondin 1 (Uniprot: Q2MKA7) comprising two furin-like domains expressed in E.coli and purified to homogeneity. Protein is lyophilised (carrier protein-free)
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Engineered mature domain of human activin A expressed in E.coli, refolded and purified to homogeneity. Optimised for extremely consistent high yield and the same bioactivity as wild-type protein. Particularly suitable for larger scale and bulk stem cell culture. Protein is lyophilised (carrier protein-free).
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Mature domain of human FGF4 (residues 79-206, Uniprot:P08620) expressed in E.coli and purified to homogeneity. Protein is in PBS at 1 mg/ml (carrier protein-free)
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Mature domain of human FGF10 (residues 64-208, Uniprot: O15520) expressed in E.coli and purified to homogeneity, Protein is in PBS at 1 mg/ml (carrier protein-free)
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Mature domain of zebrafish Danio rerio FGF2 (residues 2-154, Uniprot: B3DGE3) expressed in E.coli and purified to homogeneity as described in Ludwig et al. Nat. Methods 3, 637–646 (2006). Protein is in PBS at 2-10 mg/ml (carrier protein-free)
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Recombinant mature domain of human Activin A protein (residues 311-426, Uniprot: P08476) expressed in E.coli (animal-derived component free). Protein supplied lyophilized with no carrier protein present. Activin A is used in stem cell maintenance and differentiation.
