freeze on arrival
Lyophilized protein is stable at room temperature for 3 days, or frozen at -20°C or -80°C for 2 years.
centrifuge the vial
Before removing the lid, centrifuge to collect all the protein at the bottom of the vial. Lyophilized protein may not be visible.
add reconstitution buffer
Check product page for recommended reconstitution buffer and minimum concentration. This will help protein stability and aid maximum recovery.
Allow the protein to reconstitute for 5 minutes at room temperature.
Do not vortex. Gently agitate or pipette, avoiding foaming.
optional: add carrier protein
Addition of carrier protein reduces adsorption onto plastic and allows proteins to be stored at more dilute concentrations. 0.1-1% BSA, HSA or gelatin can be used as a carrier.
Make single use aliquots to avoid freeze/thaw cycles.
Store aliquots at -20°C or -80°C for up to 1 year.