Lyophilized Protein Reconstitution Guide

freeze proteins on arrival

freeze on arrival

Lyophilized protein is stable at room temperature for 3 days, or frozen at -20°C or -80°C for 2 years.

centrifuge the vial

Before removing the lid, centrifuge to collect all the protein at the bottom of the vial. Lyophilized protein may not be visible.

centrifuge the vial of protein
add reconstitution buffer

add reconstitution buffer

Check product page for recommended reconstitution buffer and minimum concentration. This will help protein stability and aid maximum recovery.


Allow the protein to reconstitute for 5 minutes at room temperature.

wait 5 minutes
mix gently, no foaming

mix gently

Do not vortex. Gently agitate or pipette, avoiding foaming.

optional: add carrier protein

Addition of carrier protein reduces adsorption onto plastic and allows proteins to be stored at more dilute concentrations. 0.1-1% BSA, HSA or gelatin can be used as a carrier.

optional add carrier protein to vial
prepare single use aliquots


Make single use aliquots to avoid freeze/thaw cycles.


Store aliquots at -20°C or -80°C for up to 1 year.

freeze aliquots at -20 or -80

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