Fibroblast growth factor 10 is part of the fibroblast growth factor (FGF) family. Members of this family are heparin binding glycoproteins involved in a number of different embryo and adult cell and tissue types, including mesenchymal, neuronal and epithelial cells. FGf-10 is expressed in the mesenchyme and functions through interacting with the epithelial FGF Receptor 2b (Fgfr2b) with heparin/heparan sulfate1. It has also been shown to interact weakly with FGF Receptor 1b2. The mature form of human FGF-10 is an approximately 20 kDa protein highly similar to FGF-7 and with a serine-rich region near its N-terminus3. It is secreted by mesenchymal cells and is bound and activated by extracellular FGF-BP4.
FGF-10 is first active in the limb bud mesoderm where it creates and maintains FGF signalling with epithelial FGF-8, then drives a positive feedback loop accumulating mesenchyme in the growing bud, and finally induces the apical ectodermal ridge which ultimately gives rise to feet and hands5. Lung development is based on the same epithelial-mesenchymal FGF mediations involving FGF-10 from the foregut mesenchyme signalling to FGFR-2 in the foregut epithelium6. Further, FGF-10 is involved in the development of white adipose tissue, heart, liver, brain, kidney, thymus, inner ear, tongue, trachea, eye, prostate, salivary gland, and mammary gland, and has been shown to induce migration and invasion of pancreatic cancer cells, to be associated with breast cancer risk, and patients with FGF-10 haploinsufficiency present symptoms of chronic obstructive pulmonary disease. FGF-10 also drives the differentiation of embryonic stem cells into gut-like structures, cardiomyocytes, and hepatocytes1.
Alternative protein names: Fibroblast Growth Factor-10, FGFA, KGF-2, Keratinocyte growth factor 2
- Itoh, N. & Ohta, H. Fgf10: A Paracrine-Signaling Molecule in Development, Disease, and Regenerative Medicine. Curr. Mol. Med. 14, 504–509 (2014).
- Zhang, X. et al. Receptor specificity of the fibroblast growth factor family. The complete mammalian FGF family. J. Biol. Chem. 281, 15694–700 (2006).
- Igarashi, M., Finch, P. W. & Aaronson, S. A. Characterization of recombinant human fibroblast growth factor (FGF)-10 reveals functional similarities with keratinocyte growth factor (FGF-7). J. Biol. Chem. 273, 13230–5 (1998).
- Beer, H.-D. et al. The fibroblast growth factor binding protein is a novel interaction partner of FGF-7, FGF-10 and FGF-22 and regulates FGF activity: implications for epithelial repair. Oncogene 24, 5269–77 (2005).
- Ohuchi, H. et al. The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor. Development 124, 2235–44 (1997).
- Min, H. et al. Fgf-10 is required for both limb and lung development and exhibits striking functional similarity to Drosophila branchless. Genes Dev. 12, 3156–61 (1998).
- Zhang, R.-R. et al. Stem Cell Reports Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids. Stem Cell Reports 10, 780–793 (2018).
- McCracken, K. W. et al. Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature 516, 400–404 (2014).
Summary: Mature domain of human FGF10 (residues 64-208, Uniprot: O15520) expressed in E.coli and purified to homogeneity. Mature protein is a non-glycosylated protein with a molecular mass of 17 kDa
Form: protein is provided frozen in PBS (carrier protein-free). Protein concentration is 1 mg/ml.
Molecular mass: ~17 kDa
Quality testing: all our proteins are made in-house by our scientists. We take the quality of our proteins very seriously and you can view the full quality testing data for each batch of protein by clicking on the link below
Thaw the sample on ice, spin briefly and dilute with PBS as needed. Our protein are supplied carrier-protein free. If compatible with your work, add carrier protein of your choice such as BSA, HSA or gelatin to further minimise loss by adsorption. Spin in a microfuge for 5 minutes at maximum speed, and divide the solution into suitable aliquots and store at -80°C. We recommend that single-use aliquots should be prepared to avoid freeze-thaw cycles.
Every effort is made to ensure samples are sterile however we recommend sterile filtering after dilution in media or the final working solution.