FGF22019-06-17T13:13:33+00:00

Recombinant FGF2 (bFGF)

Fibroblast growth factor 2 (FGF2) or basic FGF has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

Qkine FGF2 proteins include: 

Qk025 Recombinant human FGF-2, basic FGF, 146 aa

Qk002 Recombinant zebrafish FGF-2, basic FGF.  FGF2 from zebrafish has been used extensively to support the maintenance of human stem cells. (1–3).

Alternative protein names: Basic fibroblast growth factor, bFGF, FGF-β, FGF2, Fibroblast growth factor-basic, HBGF-2
Structure of Activin A cytokine

1. Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006).
2. Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006).
3. Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012).

Summary: Qk025 mature domain of human FGF2 (residues 144-288, Uniprot: P09038) expressed in E.coli and purified to homogeneity.

Form: protein is provided frozen in PBS (carrier protein-free)

Molecular mass: ~17 kDa

Quality testing: all our proteins are made in-house by our scientists.  We take the quality of our proteins very seriously and you can view the full quality testing data for each batch of protein by clicking on the link below

Qk025 human FGF2 batch #012

Thaw the sample on ice, spin briefly and dilute with PBS as needed.  Our protein are supplied carrier-protein free.  If compatible with your work, add carrier protein of your choice such as BSA, HSA or gelatin to further minimise loss by adsorption.  Spin in a microfuge for 5 minutes at maximum speed, and divide the solution into suitable aliquots and store at -80°C. We recommend that single-use aliquots should be prepared to avoid freeze-thaw cycles.

Every effort is made to ensure samples are sterile however we recommend sterile filtering after dilution in media or the final working solution.

Buy online or email orders@qkine.com

Summary: Qk002 mature domain of zebrafish Danio rerio FGF2 (residues 2-154, Uniprot: B3DGE3) expressed in E.coli and purified to homogeneity as described in Ludwig et al. (2).

Form: protein is provided in PBS without carrier protein at 2 mg/ml.

Molecular mass: ~17 kDa

Quality testing: all our proteins are made in-house by our scientists.  We take the quality of our proteins very seriously and you can view the full quality testing data for each batch of protein by clicking on the link below

Qk002 FGF2 batch #011

Thaw the sample on ice, spin briefly and dilute with PBS as needed.  Our protein are supplied carrier-protein free.  If compatible with your work, add carrier protein of your choice such as BSA, HSA or gelatin to further minimise loss by adsorption.  Spin in a microfuge for 5 minutes at maximum speed, and divide the solution into suitable aliquots and store at -80°C. We recommend that single-use aliquots should be prepared to avoid freeze-thaw cycles.

Every effort is made to ensure samples are sterile however we recommend sterile filtering after dilution in media or the final working solution.

Buy online or email orders@qkine.com

We take the quality of our proteins very seriously

All our proteins are produced in-house by our scientists and we understand the impact on your work if your growth factors and cytokines don't perform as expected.

Please visit this article by our founder, Marko Hyvonen to understand why we think improving growth factor quality will save valuable time and expense.

Qkine quality promise: our growth factors and cytokines work. If they don’t, we give you your money back. Simple as that.

Find out more

Buy online or email orders@qkine.com