FAQs2019-09-07T15:29:31+00:00

FAQ

Currencies accepted via our online store2020-09-30T17:04:06+00:00

We accept the following currencies via our online store:

  • GBP: Pound sterling (£)
  • EUR: Euro (€)
  • USD: United States (US) dollar (US$)
  • CAD: Canadian dollar (C$)
  • JPY: Japanese yen (¥)

To change the currency displayed on our website, simply click ‘currency’ on the top navigation bar and select your local or preferred currency from the dropdown list.

EMA/410/01-Rev3 regulation compliance2020-08-26T09:19:10+00:00

The EMA/410/01-Rev3 regulation is not directly applicable to our products as it only applies to medicinal products and the use of animal-derived products as raw materials, which we do not use in any of our manufacturing processes. However, for peace of mind, we are in the process of confirming that everything we use both directly and indirectly for manufacturing our products is compliant with the regulation.

Stability of lyophilized proteins at room temperature2020-08-26T09:17:13+00:00

We have compared our lyophilized proteins incubated at 37 °C for 3 days with those stored at -80 °C and found no difference in their bioactivity (EC50) using our standard assays. Therefore, as part of our commitment to the environment and supporting more eco-friendly packaging, we send all of our lyophilized proteins at room temperature, avoiding the need for dry ice, ice packs and gels.

How to place an order2020-08-25T17:03:01+00:00

Please order online (where you can upload a PO), email orders@qkine.com or phone +44 (0) 1223 491486 / USA toll free 1-866 877 2185.

Our team are always happy to help and if you need reserved stock from batches you have validated, bulk orders or any further information, please contact us by phone or email (orders@qkine.com)

Worldwide, fast shipping2020-09-01T11:03:11+00:00

We ship worldwide with rapid delivery, orders received before 12 pm (GMT) will be dispatched same or next day.  However, we do not ship on a Friday to prevent delivery problems over weekends.

Shipping is free for all orders over £1000 / €1000 / US$1200

Shipping and packing costs for orders < £1000 (€1000 / US$1200):

UK –  £12.50
Europe & Nordics – €30
USA & Canada – US$30
ROW – US$60

If you would like priority delivery, or would like us to ship on your courier account, please email  orders@qkine.com

Sterility of protein stock solutions2020-08-25T11:02:14+00:00

Every effort is made to ensure samples are sterile; however, we recommend sterile filtering after dilution in media or the final working solution.

Handling guidance for lyophilized cytokines including Activins, BMPs and GDF152020-08-25T11:04:42+00:00

TGF beta family proteins and other growth factors can be very poorly soluble in physiological solutions.  Please follow the handling guidance for lyophilized cytokines below to minimize loss of protein due to precipitation or adsorption to plastic.  We advise storing the recombinant protein at very low pH before dilution in cell culture media or final working solutions. Low pH will also assist in maintaining the correct disulfide structure of the protein by minimizing disulfide bond exchange reactions.

  • Resuspension in physiological buffers may cause precipitation of stock solutions, hence we recommend dissolving our lyophilized cytokines in 10 mM HCl (1:1000 dilution of concentrated HCl) while keeping the protein concentration at 50 µg/ml or above, in order to avoid loss by adsorption to plasticware.
  • To ensure you recover all of the protein, let the sample sit for a few minutes with the solubilization buffer at room temperature and pipette gently up and down (avoid foaming).
  • Rinse the tube with some more 10 mM HCl and pool with the rest.
  • The protein is tolerant of some freeze and thaw cycles, but as always with proteins, it is better to aliquot and store frozen.
  • Our proteins are supplied carrier-protein free.  If compatible with your work, add carrier protein of your choice such as BSA, HSA or gelatin to further minimize loss by adsorption.
  • Store in -80°C for long term storage. -20°C for short-term.

Every effort is made to ensure samples are sterile; however, we recommend sterile filtering after dilution in media or the final working solution.

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