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A wide range of analytical techniques can be used to measure the various properties of a protein, such as the size, charge, amino acid composition and hydrophobicity. Confirmation of the purity of growth factors by several varied techniques ensures reliability of results. When shopping for growth factors look for products where purity has been [...]
It is very hard to determine the activity of a growth factor as they have a large range of potential effects. A luciferase reporter assay is used to quantify the response of transfected cells to a growth factor by assaying the expression of luciferase (the reporter gene). As the biological activity of a growth [...]
Growth factors can be synthesised in prokaryotic cells, most commonly E. coli, or eukaryotic cells, using cell lines such as human embryonic kidney (HEK) 293 cells or Chinese hamster ovary (CHO) cells. There are benefits to each of these cell sources. Proteins produced from prokaryotic sources generally have less lot-to-lot variability and higher purity [...]
Adding a carrier protein increases protein stability, extends shelf life, allows the protein to be stored at more dilute concentrations, and helps avoid the product sticking to the walls of the vial. Consequently, adding carrier proteins can be very helpful. However, most carrier proteins are animal-derived so for animal-free and sensitive applications and assays [...]
Growth factor shelf life is influenced by many factors, including storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally speaking, growth factors are most stable when lyophilized. Our lyophilized proteins have been verified to be stable at room temperature for 3 days, or frozen for up to 2 years (either [...]
Always check the manufacturer’s instructions for how to reconstitute your lyophilized proteins (ours is here) and the product datasheet, but here are some general useful tips: Centrifuge before opening. During transportation the lyophilized protein can move around and may become lodged in the cap or on the sides of the vial. Before opening the [...]
Lyophilized (freeze dried) proteins are more stable than proteins in solution, consequently many proteins ship in lyophilized forms to allow shipping at room temperature – good for the environment and your experiments.