10 09, 2021

Do you have an ISO9001: 2015 certified quality management system?

By |2021-09-10T20:38:39+00:00September 10th, 2021||Comments Off on Do you have an ISO9001: 2015 certified quality management system?

Qkine has implemented an ISO9001:2015 compliant quality management system at its Cambridge, UK facility. Certified through BSI with certificate number FS 747253.

24 02, 2021

protein purity tests explained

By |2021-02-26T19:53:42+00:00February 24th, 2021|, |Comments Off on protein purity tests explained

A wide range of analytical techniques can be used to measure the various properties of a protein, such as the size, charge, amino acid composition and hydrophobicity. Confirmation of the purity of growth factors by several varied techniques ensures reliability of results. When shopping for growth factors look for products where purity has been [...]

24 02, 2021

What is a luciferase reporter assay and how does it measure bioactivity?

By |2021-02-26T19:55:33+00:00February 24th, 2021|, |Comments Off on What is a luciferase reporter assay and how does it measure bioactivity?

It is very hard to determine the activity of a growth factor as they have a large range of potential effects. A luciferase reporter assay is used to quantify the response of transfected cells to a growth factor by assaying the expression of luciferase (the reporter gene). As the biological activity of a growth [...]

24 02, 2021

Growth factor source; why should I care?

By |2021-02-26T19:58:44+00:00February 24th, 2021|, |Comments Off on Growth factor source; why should I care?

Growth factors can be synthesised in prokaryotic cells, most commonly E. coli, or eukaryotic cells, using cell lines such as human embryonic kidney (HEK) 293 cells or Chinese hamster ovary (CHO) cells. There are benefits to each of these cell sources. Proteins produced from prokaryotic sources generally have less lot-to-lot variability and higher purity [...]

24 02, 2021

Carrier vs carrier- protein free? When would/wouldn’t I want a carrier protein?

By |2021-02-26T20:16:51+00:00February 24th, 2021||Comments Off on Carrier vs carrier- protein free? When would/wouldn’t I want a carrier protein?

Adding a carrier protein increases protein stability, extends shelf life, allows the protein to be stored at more dilute concentrations, and helps avoid the product sticking to the walls of the vial. Consequently, adding carrier proteins can be very helpful. However, most carrier proteins are animal-derived so for animal-free and sensitive applications and assays [...]

24 02, 2021

How to store growth factors

By |2021-02-26T20:18:15+00:00February 24th, 2021||Comments Off on How to store growth factors

Growth factor shelf life is influenced by many factors, including storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally speaking, growth factors are most stable when lyophilized.  Our lyophilized proteins have been verified to be stable at room temperature for 3 days, or frozen for up to 2 years (either [...]

24 02, 2021

General tips for reconstituting lyophilized growth factors

By |2021-02-26T20:19:09+00:00February 24th, 2021|Comments Off on General tips for reconstituting lyophilized growth factors

Always check the manufacturer’s instructions for how to reconstitute your lyophilized proteins (ours is here) and the product datasheet, but here are some general useful tips: Centrifuge before opening. During transportation the lyophilized protein can move around and may become lodged in the cap or on the sides of the vial. Before opening the [...]

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