Human BMP2 Qk007 protein purity SDS-PAGE lot #010

Recombinant human/mouse/rat/bovine/porcine BMP-2 protein (Qk007)

Name: Recombinant human/mouse/rat/bovine/porcine BMP-2 protein (Qk007)
SKU: Qk007
Availability: InStock

Human/mouse/rat/bovine/porcine BMP-2 protein (bone morphogenetic protein 2) protein is member of the TGFβ family and a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.

26 kDa disulfide–linked bioactive highly pure dimer comprised of the mature domain of human BMP-2 protein. Animal-free (AOF) and carrier protein-free.

Orders are typically shipped same or next day (except Friday).
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1000µg will be despatched as 2 x 500µg

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Description

Summary

High purity human/mouse/rat/bovine/porcine bone morphogenetic protein 2, BMP2 (Uniprot: P12643).
>98%, by SDS-PAGE quantitative densitometry
26 kDa (dimer)
Expressed in E. coli
Animal-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Handling and storage FAQ

Featured applications

Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes

Alternative protein names

Bone morphogenetic protein 2,BDA2, SSFSC, BMP-2A, BMP-2, Bone morphogenetic protein 2A

Background references

Species reactivity

Human, mouse, rat, bovine, porcine

Frequently used together

Recombinant human activin A protein
Recombinant human FGF2 145aa protein

Bioactivity

BMP-2 activity is determined using the BMP2-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of BMP2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.

EC50 = 0.3 nM (7.8 ng/mL).

Data from Qk007 lot #10.

Purity

BMP2 migrates as a single band at 26 kDa in non-reducing (NR) conditions and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk007 lot #010

Further quality assays

Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%

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Download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Bone morphogenetic protein 2 (BMP2) is a member of the BMP subgroup of the transforming growth factor beta (TGF-β) superfamily. It plays numerous roles in the developing embryo, such as embryonic patterning along the dorso-ventral axis, organogenesis, limb bud formation, and bone and cartilage growth. BMP2 protein is a potent differentiation factor and directs human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal and neural lineages, and chondrocytes¹. Recombinant BMP2 protein induces bone and cartilage formation in vitro and chondrogenesis in human adult mesenchymal stem cells².

Human bone morphogenetic protein 2 (BMP2) is synthesized as a preproprotein consisting of N-terminal signal peptide, 259 amino acid residue pro-domain and 114 residue mature domain. Proteolytic removal of the propeptide enables mature BMP2 protein to form active disulfide-linked homodimers, and heterodimers with BMP7.

Mature human BMP2 protein shares 100% amino acid sequence identity with mouse and rat BMP2. It also shares 85% amino acid sequence identity with the related protein, BMP4, and less than 51% identity with other BMPs.

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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