FGF-4 (human)

£160.00£1,200.00

Mature domain of human FGF4 (residues 79-206, Uniprot:P08620) expressed in E.coli and purified to homogeneity.  Mature protein is a non-glycosylated protein with a molecular weight of ca. 14 kDa

SKU: Qk004_hFGF4 Categories: , Tags: , , ,

Description

Fibroblast growth factor 4 (FGF-4) is a member of the FGF superfamily with a physiological role in the regulation of proliferation and differentiation in embryonic stem cells and tissue stem cells 1–3

FGF-4 has been shown to promote neural stem cell proliferation and neuronal differentiation in the postnatal brain 4, increase the proliferation rate of human adult bone-marrow derived mesenchymal stem cells 5 and support the maintenance, proliferation and self-renewal properties of human embryonic stem cells 6

Synergism between FGF4 and WNT signalling acts to form hindgut organoids from iPSC-derived human posterior gut endoderm cells7 and in FGF4 can be used in culture systems to mimic embryonic intestinal development during directed differentiation of pluripotent stem cells into intestinal organoids8.

Product: recombinant human FGF4

Mature domain of human FGF4 (residues 79-206, Uniprot:P08620) expressed in E.coli and purified to homogeneity.  Mature protein is a non-glycosylated protein with a molecular weight of ca. 14 kDa

Protein is provided in PBS without carrier protein at 1 mg/ml.

Sequence:

M G I K R L R R L Y C N V G I G F H L Q A L P D G R I G G A H A D T R D S L L E L S P V E R G V V S I F G V A S R F F V A M S S K G K L Y G S P F F T D E C T F K E I L L P N N Y N A Y E S Y K Y P G M F I A L S K N G K T K K G N R V S P T M K V T H F L P R L

Alternative names:
Fibroblast Growth Factor-4, Kaposi’s sarcoma-associated FGF, k-FGF, Heparin secretory-transforming protein 1 HST-1, Transforming protein KS3, Heparin-binding growth factor 4, HBGF-4

Quality control:
We take the quality of our products very seriously, please see this blog by our founder, Marko Hyvonen to understand why we think improving growth factor quality will save valuable R&D time and expense. All our proteins are produced in-house by our scientists and we understand the potential impact on your work if your growth factors and cytokines are not consistent and of the highest quality.  We provide quality control data for each individual batch, please contact us any time if you have any questions.

The biochemical identity of each batch of human recombinant FGF4 is testing by SDS-PAGE and mass spec.  Correct recovery from the vial is checked to ensure you will get what you expect.

Endotoxin testing:
Stem cell cultures are sensitive to endotoxins, which can be present in media, serum and as a contaminants on plastic ware. We optimise our protein production processes to ensure the lowest possible levels of endotoxin contamination.  Endotoxin levels are determined by an external expert microbiological testing services provider and we set our endotoxin pass criteria at the industry leading <0.1 EU per ug protein and aim for <0.01 EU per ug protein.  We report the exact endotoxin levels for each batch.

hFGF2 batch #011 endotoxin level = <0.0005 EU/ug protein (below the detection level of the assay) 

Bioactivity:
Our human recombinant FGF4 has been extensively tested by an independent stem cell specialist biotech company, Stemnovate.

Please see the datasheet for full batch-specific quality testing results, customer feedback and additional information.

Human recombinant FGF4 Qk004 batch #010 datasheet

Please contact us at orders@qkine.com for batch reservation, bulk purchasing arrangements or any other questions.

References:

  1. Beenken, A. & Mohammadi, M. The FGF family: biology, pathophysiology and therapy. Nat. Rev. Drug Discov. 8, 235–53 (2009).
  2. Coutu, D. L. & Galipeau, J. Roles of FGF signaling in stem cell self-renewal, senescence and aging. Aging (Albany. NY). 3, 920–33 (2011).
  3. Kosaka, N., Sakamoto, H., Terada, M. & Ochiya, T. Pleiotropic function of FGF-4: Its role in development and stem cells. Dev. Dyn. 238, 265–276 (2009).
  4. Kosaka, N. et al. FGF-4 regulates neural progenitor cell proliferation and neuronal differentiation. FASEB J. 20, 1484–1485 (2006).
  5. Farré, J. et al. FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells. Growth Factors 25, 71–76 (2007).
  6. Mayshar, Y. et al. Fibroblast Growth Factor 4 and Its Novel Splice Isoform Have Opposing Effects on the Maintenance of Human Embryonic Stem Cell Self-Renewal. Stem Cells 26, 767–774 (2008).
  7. Zhang, R.-R. et al. Stem Cell Reports Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids. Stem Cell Reports 10, 780–793 (2018).
  8. McCracken, K. W. et al. Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature 516, 400–404 (2014).

Additional information

Unit size

1000 µg, 500 µg, 100 µg, 50 µg

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