FGF-2, basic FGF (human) 146 aa

£100.00£600.00

Mature domain of human FGF2 (residues 144-288, Uniprot: P09038) expressed in E.coli and purified to homogeneity.  Mature protein is a non-glycosylated protein with a molecular weight of ca. 17 kDa.

Protein is provided in PBS without carrier protein at 1.4 mg/ml.

SKU: Qk025_hFGF2 Categories: , Tags: , , ,

Description

Fibroblast growth factor 2 (FGF2) or basic FGF has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.  This is the slightly truncated form of the protein, used routinely in many culture systems.

Product: recombinant human FGF2, basic FGF (146 aa)

Mature domain of human FGF2 (residues 144-288, Uniprot: P09038) expressed in E.coli and purified to homogeneity.  Mature protein is a non-glycosylated protein with a molecular weight of ca. 17 kDa.

Protein is provided in PBS without carrier protein at 1.4 mg/ml.

Sequence:

M A L P E D G G S G A F P P G H F K D P K R L Y C K N G G F F L R I H P D G R V D G V R E K S D P H I K L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K S

Alternative names:
Basic fibroblast growth factor, bFGF, FGF-β, FGF2, Fibroblast growth factor-basic, HBGF-2

Quality control:
We take the quality of our products very seriously, please see this blog by our founder, Marko Hyvonen to understand why we think improving growth factor quality will save valuable R&D time and expense. All our proteins are produced in-house by our scientists and we understand the potential impact on your work if your growth factors and cytokines are not consistent and of the highest quality.  We provide quality control data for each individual batch, please contact us any time if you have any questions.

The biochemical identity of each batch of human recombinant FGF2 is testing by SDS-PAGE and mass spec.  Correct recovery from the vial is checked to ensure you will get what you expect.

Endotoxin testing:
Stem cell cultures are sensitive to endotoxins, which can be present in media, serum and as a contaminants on plastic ware. We optimise our protein production processes to ensure the lowest possible levels of endotoxin contamination.  Endotoxin levels are determined by an external expert microbiological testing services provider and we set our endotoxin pass criteria at the industry leading <0.1 EU per ug protein and aim for <0.01 EU per ug protein.  We report the exact endotoxin levels for each batch.

hFGF2 batch #011 endotoxin level = 0.0068 EU/ug protein

Bioactivity:
Our human recombinant FGF2 has been extensively tested by an independent stem cell specialist biotech company, Stemnovate and benchmarked against other commercial sources.  Our academic customers also report excellent results using our FGF2.

Please see the datasheet for full batch-specific quality testing results, customer feedback and additional information.

Human recombinant FGF2 Qk025 batch #011 datasheet

Please contact us at orders@qkine.com for batch reservation, bulk purchasing arrangements or any other questions.

Additional information

Unit size

1000 µg, 500 µg, 100 µg, 50 µg

1 review for FGF-2, basic FGF (human) 146 aa

  1. Admin

    “Just to let you know, I’ve tested the GFs and the Activin A and human FGF worked exceptionally well compared to what I usually use”. Keele, UK.

    A 14-day directed differentiation protocol was applied to human embryonic stem cells in order to generate chondroprogenitors. The use of Qkine human Activin-A and human FGF resulted in significantly higher levels of ACAN, COL2 and SOX9 gene expression and significantly lower levels of OCT4 gene expression.

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