Activin A 2.1 (human) optimised by Qkine

£180.00£1,900.00

Engineered mature domain of human activin A (Uniprot: P08476) expressed in E.coli, refolded and purified to homogeneity. This engineered form of Activin A incorporates an N-terminal truncation to remove a disulphide-linked extension. The resulting protein is optimised for extremely consistent high yield with no observed changes in bioactivity compared to the wild-type. This form is particularly suitable for larger scale culture processes. The mature engineered protein is a disulphide-linked dimer with a molecular weight of 24 kDa.

Description

Activin A is a member of the TGFβ superfamily of growth factors involved in stem cell differentiation and maintenance, regulation of embryogenesis, development of the reproductive system, wound healing and regulation of immune responses. The activity of Activin A is regulated by the high-affinity inhibitor, follistatin (1), and inhibins.
Activins are disulphide-linked homo- and heterodimers of four inhibin β chains. The best characterised are Activin A and Activin B, homodimers of inhibin βA and inhibin βB respectively. Activins, like all other members in the TGF-β superfamily, are synthesized as larger precursors consisting of an N-terminal signal peptide, a pro-domain of 250–350 residues and a highly conserved mature domain. The pro-domain, which is cleaved off in the mature protein, has important roles in the biosynthesis, stabilisation, transportation and signalling of the growth factors (2).
Activin A is used for maintenance of pluripotency in many human induced-pluripotent stem cell and human embryonic stem cell lines (3), and to induce stem cell differentiation into endoderm (4) and other cell fates.

Product: Qk005 ActA 2.1: engineered recombinant human Activin A
Engineered mature domain of human activin A (Uniprot: P08476) expressed in E.coli, refolded and purified to homogeneity. This engineered form of Activin A incorporates an N-terminal truncation to remove a disulphide-linked extension. The resulting protein is optimised for extremely consistent high yield with no observed changes in bioactivity compared to the wild-type. This form is particularly suitable for larger scale culture processes. The mature engineered protein is a disulphide-linked dimer with a molecular weight of 24 kDa.

Protein is provided in lyophilised form without carrier protein.

Alternative names (wild-type protein):
ActA, Activin beta-A chain, Erythroid differentiation protein (EDP), Follicle-Stimulating Hormone (FSH) releasing protein (FRP), INHBA, Inhibin beta A, Inhibin beta 1

Quality control:
We take the quality of our products very seriously, please see this blog by our founder, Marko Hyvonen to understand why we think improving growth factor quality will save valuable R&D time and expense. All our proteins are produced in-house by our scientists and we understand the potential impact on your work if your growth factors and cytokines are not consistent and of the highest quality.  We provide quality control data for each individual batch, please contact us any time if you have any questions.

The biochemical identity of each batch of human recombinant ActA 2.1 is testing by SDS-PAGE and mass spec.  Correct recovery from the vial is checked to ensure you will get what you expect.

Endotoxin testing:
Stem cell cultures are sensitive to endotoxins, which can be present in media, serum and as a contaminants on plastic ware. We optimise our protein production processes to ensure the lowest possible levels of endotoxin contamination.  Endotoxin levels are determined by an external expert microbiological testing services provider and we set our endotoxin pass criteria at the industry leading <0.1 EU per ug protein and aim for <0.01 EU per ug protein.  We report the exact endotoxin levels for each batch.

hActA 2.1 batch #010 endotoxin level < 0.005 EU/ug protein (below detection level of the assay)

Bioactivity:
Our engineered human recombinant Activin A has been extensively tested and shows comparable bioactivity to wild-type Activin A.

Please see the datasheet for full batch-specific quality testing results, customer feedback and additional information.

Human recombinant Activin A 2.1 engineered Qk005 batch #011 datasheet

Please contact us at orders@qkine.com for batch reservation, bulk purchasing arrangements or any other questions.

 

References:
1. Harrington, A. E. et al. Structural basis for the inhibition of activin signalling by follistatin. EMBO J. 25, 1035–1045 (2006).
2. Wang, X., Fischer, G. & Hyvönen, M. Structure and activation of pro-activin A. (2016). doi:10.1038/ncomms12052
3. Pauklin, S. & Vallier, L. Activin/Nodal signalling in stem cells. Development 142, 607–19 (2015).
4. D’Amour, K. A. et al. Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat. Biotechnol. 23, 1534–1541 (2005).

 

We take the quality of our products very seriously. All our proteins are produced in-house by our scientists and we understand the potential impact on your work if your growth factors and cytokines are not consistent and of the highest quality.  We provide quality control data for each individual batch, please contact us any time if you have any questions.

 

Additional information

Unit size

1000 µg, 500 µg, 100 µg, 50 µg, 3000 µg

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